New method for identification and determination of ibotenic acid, muscimol.
The CE–ESI-MS/MS (capillary electrophoresis coupled with electrospraytandem mass spectrometry) method for the identification, separation and determination of mushroom toxins, namely ibotenic acid, muscimol and muscarine, was developed. It proved to be sensitive and thus useful for the real sample analysis with omitting the labor and time consuming pretreatment step. The CE–ESI-MS/MS method was applied on the spiked human urine. The analytical characteristics of the proposed method, such as limits of detection, linearity and repeatability of the peak area and the migration time, were evaluated. Developed method is sufficient for the determination and quantification of studied toxins in human urine after mushroom intoxication.
